Calpain 1 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88205

Novus Biologicals, part of Bio-Techne
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NBP1-88205
NBP1-88205-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Calpain 1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PDALKSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCSFVLALMQKHRRRERRFGRDMETIGFAVYEVPPELVGQPAVHLKRDFFLANASRARSEQFINLREVSTRFRLPPGEYVVVPSTFEPNKE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (88%), Mouse reactivity reported in the scientific literature (PMID: 30154452)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Calpain 1 Antibody - BSA Free

Calpain 1 Antibody - BSA Free Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody - BSA Free [NBP1-88205]

Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody - BSA Free [NBP1-88205]

Staining of human cell line A-431 shows localization to cytosol.
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205] - Staining in human fallopian tube and testis tissues using anti-CAPN1 antibody. Corresponding CAPN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205]

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP1-88205] - Staining of human fallopian tube shows high expression.
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Applications for Calpain 1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
WB reported in scientific literature (PMID: 30154452). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Calpain 1

The calpain (calcium-dependent protease or calcium-activated neutral protease) system consists of two ubiquitous forms of calpain (Mu Calpain and M Calpain), a tissue specific calpain (N Calpain), and a calpain inhibitory protein (calpastatin). The calpain system has been detected in every vertebrate tissue examined, and has been suggested to play a regulatory role in cellular protein metabolism. This regulatory role may have important implications in platelet aggregation and pathologies associated with altered calcium homeostasis and protein metabolism such as ischemic cell injury and degenerative diseases. Inhibitors of calpain have been shown to block dexamethasone and low-level irradiation induced apoptosis in thymocytes suggesting that calpain has a regulatory or mechanistic role in apoptotic cell death.Mu and M Calpains are heterodimers consisting of 28 kDa and 80 kDa subunits. The 28 kDa subunit is identical in the two isoforms, but the 80 kDa subunits differ with ~50% sequence similarity. 28 kDa / 80 kDa complexes are thought to be inactive proenzymes which, upon binding of calcium, undergo conformational changes that promotes cleavage of the 28 kDa subunit and results in enzyme activation.

Long Name

Calpain-1 catalytic subunit

Alternate Names

Calpain mu-type, CANP 1, CANPL1, CAPN1, EC 3.4.22.52, Micromolar-calpain, muCANP, PIG30

Gene Symbol

CAPN1

UniProt

P07384 (Human)

Additional Calpain 1 Products

Product Documents for Calpain 1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Calpain 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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