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Cadherin-13 Antibody (OTI3H6), Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP2-02528

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NBP2-02528

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H6

Concentration

0.41 mg/ml

Product Summary for Cadherin-13 Antibody (OTI3H6)

Immunogen

Full length human recombinant protein of human CDH13 (NP_001248) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

75.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cadherin-13 Antibody (OTI3H6)

Western Blot: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Analysis of extracts (10ug) from 10 Human tissue by using anti-CDH13 monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunocytochemistry/Immunofluorescence: Cadherin-13 Antibody (OTI3H6) [NBP2-02528] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Cadherin-13 Antibody (3H6)
Western Blot: Cadherin-13 Antibody (3H6) [NBP2-02528] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Cadherin-13 Antibody (3H6 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti- Cadherin-13 Antibody (3H6)

Applications for Cadherin-13 Antibody (OTI3H6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.41 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cadherin-13

This gene is a member of the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region but, unlike the typical cadherin superfamily member, lacks the highly conserved cytoplasmic region. This particular cadherin is a putative mediator of cell-cell interaction in the heart and may act as a negative regulator of neural cell growth. The gene locus is hypermethylated or deleted in breast, ovarian and lung cancers. Two major mRNA transcripts encoding identical proteins are found, products of alternative polyadenylation sites

Alternate Names

Cadherin13, CDH13, H-Cadherin, T-Cadherin

Entrez Gene IDs

1012 (Human)

Gene Symbol

CDH13

Additional Cadherin-13 Products

Product Documents for Cadherin-13 Antibody (OTI3H6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Cadherin-13 Antibody (OTI3H6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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