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Key Product Details

Species Reactivity

Human, Porcine, Baboon, Bacteria, Equine

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # aE11

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for C5b-9 Antibody (aE11) - BSA Free

Immunogen

Purified C5b-9

Epitope

Binds to a neoepitope exposed on C9.

Reactivity Notes

Use in Bacteria reported in scientific literature (PMID:32900809).

Specificity

Binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for C5b-9 Antibody (aE11) - BSA Free

Immunohistochemistry-Frozen: C5b-9 Antibody (aE11) - BSA Free [NBP1-05120] - Vascular endothelia and dendritic cells of germinal center have strong and intensive label. Dilution: 1:10.

Applications for C5b-9 Antibody (aE11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500
Application Notes
Use in Flow Cytometry reported in scientific literature (PMID:32900809).ELISA: NBP1-05120 is well suited for quantifying TCC in ELISA. WB: NBP1-05120 is not recommended for Western blotting as the epitope is destroyed during the process. IHC: TCC in various tissues by immunohistochemistry (frozen and paraffin sections). Use in ICC/IF reported in scientific literature (PMID: 27432952).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 6 publications using NBP1-05120 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

15mM Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: C5b-9

C5b-9 is also known as the terminal complement complex (TCC). The TCC consists of C5b, C6, C7, C8 and C9 and forms the membrane attack complex (MAC) as well as the non-lytic fluid-phase SC5b-9 complex (with protein S). The MAC forms channels in target cell membranes leading to cell lysis by osmotic leakage. The complexes contain neoantigens that are absent from the individual native components from which they are formed and this antibody is directed against a neoepitope exposed on C9 when incorporated into the TCC (1,2).

Alternate Names

MAC, Membrane attack complex, sC5-b9, sC5b-9, TCC, Terminal complement complex

Product Documents for C5b-9 Antibody (aE11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for C5b-9 Antibody (aE11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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