C. botulinum BoNT-E Light Chain Alexa Fluor™ Plus 488-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5424AFP488
Conjugate
Catalog #
Key Product Details
Species Reactivity
C. botulinum
Applications
Western Blot
Label
Alexa Fluor Plus 488 (Excitation = 493 nm, Emission = 518 nm)
Antibody Source
Polyclonal Goat IgG
Product Specifications
Specificity
Detects C. botulinum BoNT-E Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with rBoNT-B LC is observed and less than 1% cross-reactivity with rBoNT-C HCR and rBoNT-D HCR is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Background: BoNT-E Light Chain
Botulinum Neurotoxin Type E is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2‑4). E. coli-expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.
References
- Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
- Montecucco, C. and Giampietro, S. (1993) Trends. Biochem. Sci. 18:324.
- Turton, K. et al. (2002) Trends. Biochem. Sci. 27:552.
- Schiavo, G. et al. (2000) Physiol. Rev. 80:717.
Long Name
Botulinum Neurotoxin Type E Light Chain
Alternate Names
BoNTE Light Chain
UniProt
Additional BoNT-E Light Chain Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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