Detects C. botulinum BoNT-B Light Chain in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant Light Chains of BoNT-A, -E, or recombinant Heavy Chains of BoNT-C, or -D is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Applications for C. botulinum BoNT-B Light Chain Antibody

Application

Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Botulinum Neurotoxin Type B Light Chain (Catalog # 5420-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Botulinum Neurotoxin Type B Light Chain (Catalog # 5420-ZN)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-B Light Chain

Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4). E. coli expressed recombinant light chains are active proteases. In the absence of the heavy chains, however, they lack toxicity because they cannot enter into host cells.

References

Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Long Name

Botulinum Neurotoxin Type B Light Chain

Alternate Names

BoNTB Light Chain

Entrez Gene IDs

6030102 (C. botulinum)

Gene Symbol

BONT/B

UniProt

P10844 (C. botulinum)

Additional BoNT-B Light Chain Products

Product Documents for C. botulinum BoNT-B Light Chain Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for C. botulinum BoNT-B Light Chain Antibody

For research use only

Citations
Reviews

C. botulinum BoNT-B Light Chain Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5420

Key Product Details

Species Reactivity

Validated:

Cited:

Applications