C. botulinum BoNT-B Light Chain Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB5420

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MAB5420
MAB5420-SP
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Key Product Details

Species Reactivity

C. botulinum

Applications

Validated:

Western Blot

Cited:

Surface Plasmon Resonance (SPR)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 579631

View all BoNT-B Light Chain Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Clostridium BoNT-B Light Chain
Pro2-His428
Accession # P10844

Specificity

Detects C. botulinum BoNT-B Light Chain in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant Light Chains of BoNT-A, -C1, -D, -E, -F, or -G is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for C. botulinum BoNT-B Light Chain Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Botulinum Neurotoxin Type B Light Chain (Catalog # 5420-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BoNT-B Light Chain

Botulinum Neurotoxin Type B is one of the seven serotypes of Botulinum Neurotoxins (BoNTs) produced by various strains of Clostridium botulinum (1, 2). BoNTs are synthesized as inactive single chain protein precursors and activated by proteolytic cleavage to generate disulfide-linked two-chain proteins. The 50 kDa light chain contains the catalytic domain, whereas the 100 kDa heavy chain contains an internal translocation domain and a receptor binding domain (3). BoNTs are the most potent protein toxins for humans. As zinc proteases, they cleave SNARE proteins to elicit flaccid paralysis in botulism by blocking acetylcholine release at the neuromuscular junction (2-4).

References

  1. Campbell K.D. et al. (1993) J. Clin. Microbiol. 31:2255.
  2. Montecucco, C. and Giampietro, S. (1993) Trends Biochem. Sci. 18:324.
  3. Turton, K. et al. (2002) Trends Biochem. Sci. 27:552.
  4. Schiavo, G. et al. (2000) Physiol. Rev. 80:717.

Long Name

Botulinum Neurotoxin Type B Light Chain

Alternate Names

BoNTB Light Chain

Entrez Gene IDs

6030102 (C. botulinum)

Gene Symbol

BONT/B

UniProt

P10844 (C. botulinum)

Additional BoNT-B Light Chain Products

Product Documents for C. botulinum BoNT-B Light Chain Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for C. botulinum BoNT-B Light Chain Antibody

For research use only

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