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BTN3A2 Antibody (3G2) - Chimeric

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48392

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48392-100ug
NBP3-48392-10ug

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Human IgG1 Clone # 3G2

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

BTN3A2 recombinant protein expressed in mammalian cells (Accession# P78410). The exact amino acid sequence is proprietary.

Clonality

Monoclonal

Host

Human

Isotype

IgG1

Description

This product is shipped at ambient temperature. Store at -20C to -80C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80C (Avoid repeated freezing and thawing).

Scientific Data Images for BTN3A2 Antibody (3G2) - Chimeric

BTN3A2 Antibody (3G2) - Chimeric

Flow Cytometry: BTN3A2 Antibody (3G2) - Chimeric [NBP3-48392]

Flow cytometry analysis of antigen binding of anti-human BTN3A2 mAb(NBP3-48392). (A) NBP3-48392 does not bind to MCF-7 cells that do not express BTN3A2. (B) A clear peak shift of NBP3-48392 was seen compared to the control when incubated with BTN3A2-expressing Raji cells, indicating strong binding of NBP3-48392 to BTN3A2. Antibodies were incubated at 5 ug/mL.
BTN3A2 Antibody (3G2) - Chimeric

Flow Cytometry: BTN3A2 Antibody (3G2) - Chimeric [NBP3-48392]

Flow cytometry analysis with Anti-BTN3A2 (3G2) mAb on HEK293 cells transfected with human BTN3A2 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
BTN3A2 Antibody (3G2) - Chimeric

Flow Cytometry: BTN3A2 Antibody (3G2) - Chimeric [NBP3-48392]

Flow cytometry analysis of antigen binding of anti-human BTN3A2 mAb(NBP3-48392). (A) NBP3-48392 does not bind to MCF-7 cells that do not express BTN3A2. (B) A clear peak shift of NBP3-48392 was seen compared to the control when incubated with BTN3A2-expressing 8226 cells, indicating strong binding of NBP3-48392 to BTN3A2. Antibodies were incubated at 5 ug/mL.

Applications for BTN3A2 Antibody (3G2) - Chimeric

Application
Recommended Usage

Flow Cytometry

1:100

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified from cell culture supernatant

Reconstitution

Dissolve the lyophilized antibody in sterile and low endotoxin water. The recommend volume of water is lot specific. Please refer to the instructions printed on the product vial or contact technical support.

Formulation

PBS, pH 7.4, 5-8% Trehalose

Preservative

Trehalose

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in powder form. Store at -80C once reconstituted.

Background: BTN3A2

BTN3A2 encodes a member of the immunoglobulin superfamily, containing two Ig domains with similarity to Ig variable and Ig constant domains. The gene resides in the juxta-telomeric region of the major histocompatability class 1 locus on chromosome 6 in the seven member BTN cluster, which includes butyrophilin, and three members each of the BTN2 and BTN3 subfamilies. Alternatively spliced transcript variants have been described but their full-length nature has yet to be determined.

Long Name

Butyrophilin, Subfamily 3, Member A2

Alternate Names

BT3.2, BT3.3, BTF4

Gene Symbol

BTN3A2

Additional BTN3A2 Products

Product Documents for BTN3A2 Antibody (3G2) - Chimeric

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BTN3A2 Antibody (3G2) - Chimeric

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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