BRIP1/FANCJ Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31883
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Proximity Ligation Assay
Cited:
Western Blot, Proximity Ligation Assay
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BRIP1/FANCJ. The exact sequence is proprietary.
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 26490168).
Localization
Nucleus
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
141 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit BRIP1/FANCJ Antibody - BSA Free (NBP1-31883) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-BRIP1/FANCJ Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for BRIP1/FANCJ Antibody - BSA Free
![Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]](https://resources.bio-techne.com/images/products/BRIP1-FANCJ-Antibody-Western-Blot-NBP1-31883-img0017.jpg "Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]")
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883]
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.![Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]](https://resources.bio-techne.com/images/products/BRIP1-FANCJ-Antibody-Immunohistochemistry-Paraffin-NBP1-31883-img0014.jpg "Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]")
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883]
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - SAS xenograft. FANCJ antibody [N1N2], N-term dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Applications for BRIP1/FANCJ Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Proximity Ligation Assay
Reported in scientific literature (PMID:33445471)
Western Blot
1:500-1:3000
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS, 20% Glycerol
Format
BSA Free
Preservative
0.01% Thimerosal
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: BRIP1/FANCJ
Long Name
BRCA1 Interacting Protein C-terminal Helicase 1
Alternate Names
BACH1, FACJ, FANCJ
Gene Symbol
BRIP1
Additional BRIP1/FANCJ Products
Product Documents for BRIP1/FANCJ Antibody - BSA Free
Product Specific Notices for BRIP1/FANCJ Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information go to www.P65Warnings.ca.gov.