Brg1 Antibody (24GB2065)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51540
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB2065
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Brg1 (Uniprot: P51532)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Brg1 Antibody (24GB2065)
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (24GB2065)[NBP3-51540] -
Brg1 antibody (1:1,000), Top panel: wild-type (WT); Bottom panal: SMARCA4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SMARCA4 was stained magenta with Alexa Fluor(R) 647. Scale bar, 20 um.
Immunocytochemistry/ Immunofluorescence: Brg1 Antibody (24GB2065)[NBP3-51540] -
Immunocytochemical staining of HepG2 cells with Brg1 antibody (1:1,000). Nuclei were stained blue with DAPI; Brg1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 um.
Flow Cytometry: Brg1 Antibody (24GB2065)[NBP3-51540] -
Validation of Brg1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Brg1 antibody (1:2,000) and analyzed using BD flow cytometer.Applications for Brg1 Antibody (24GB2065)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Brg1
Long Name
BRM/SWI2-related gene 1
Alternate Names
SMARCA4, SNF2, SNF2-beta, SNF2L4, SNF2LB, SWI2
Gene Symbol
SMARCA4
Additional Brg1 Products
Product Documents for Brg1 Antibody (24GB2065)
Product Specific Notices for Brg1 Antibody (24GB2065)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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