Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for BRD7 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 601 and 651 of human bromodomain containing 7 using the numbering given in entry NP_037395.2 (GeneID 29117).

Predicted Species

Orangutan (100%). Backed by our 100% Guarantee.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BRD7 Antibody

Western Blot: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HEK293T, HeLa, LNCaP, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRD7 (NBP1-28727) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: BRD7 Antibody [NBP1-28727] - Detection of human BRD7 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BRD7 antibody NBP1-28727 (lot NBP1-28727-2) used for IP at 6 ug per reaction. BRD7 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-28727-1). For blotting immunoprecipitated BRD7, NBP1-28727 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for BRD7 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 1 publication using NBP1-28727 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRD7

Bromodomain-containing protein 7 was identified in a two-hybrid screen as an interactor of interferon regulatory factor-2 (IRF-2) and called CELTIX1. BRD7 was also identified as a binding partner of disheveled-1 (Dvl-1) and found to play a role in Wnt signaling and the regulation of glycogen synthase kinase 3-beta activity. Recently, BRD7 was identified as a subunit of the polybromo-associated BRG1-associated factor (PBAF) complex and shown to be required for the modulation of transcription in embryonic stem cells.

Long Name

Bromodomain Containing 7

Alternate Names

BP75, CELTIX1, NAG4

Entrez Gene IDs

29117 (Human)

Gene Symbol

BRD7

UniProt

Additional BRD7 Products

Product Documents for BRD7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRD7 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Loading...
Loading...