BRD4 Antibody (CL1115) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-52958

Novus Biologicals, part of Bio-Techne
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NBP2-52958-25ul
NBP2-52958
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Conjugate
Catalog #
Alexa Fluor 350
NBP3-44012AF350
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Alexa Fluor 405
NBP3-44012AF405
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Alexa Fluor 488
NBP3-44012AF488
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Alexa Fluor 532
NBP3-44012AF532
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Alexa Fluor 594
NBP3-44012AF594
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Alexa Fluor 647
NBP3-44012AF647
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Alexa Fluor 700
NBP3-44012AF700
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Alexa Fluor 750
NBP3-44012AF750
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Alexa Fluor Plus 488
NBP3-44012AFP488
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Alexa Fluor Plus 555
NBP3-44012AFP555
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Alexa Fluor Plus 594
NBP3-44012AFP594
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Alexa Fluor Plus 647
NBP3-44012AFP647
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Alexa Fluor Plus 680
NBP3-44012AFP680
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Allophycocyanin
NBP3-44012APC
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Biotin
NBP3-44012B
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CoraFluor 1
NBP3-44012CL1
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DyLight 350
NBP3-44012UV
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DyLight 405
NBP3-44012V
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DyLight 488
NBP3-44012G
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DyLight 550
NBP3-44012R
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DyLight 594
NBP3-44012DL594
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DyLight 650
NBP3-44012C
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DyLight 680
NBP3-44012FR
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DyLight 755
NBP3-44012IR
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FITC
NBP3-44012F
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HRP
NBP3-44012H
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Janelia Fluor 525
NBP3-44012JF525
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Janelia Fluor 549
NBP3-44012JF549
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Janelia Fluor 646
NBP3-44012JF646
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Janelia Fluor 669
NBP3-44012JF669
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mFluor Violet 450 SE
NBP3-44012MFV450
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mFluor Violet 500 SE
NBP3-44012MFV500
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mFluor Violet 610 SE
NBP3-44012MFV610
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PE
NBP3-44012PE
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PerCP
NBP3-44012PCP
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL1115

Format

BSA Free

Concentration

1 mg/ml

View all BRD4 Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from O60885, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for BRD4 Antibody (CL1115) - BSA Free

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958]

Western Blot: BRD4 Antibody (CL1115) [NBP2-52958] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-BRD4 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/ Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958]

Immunocytochemistry/Immunofluorescence: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of MCF-7 cells using the Anti-BRD4 monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958]

Immunohistochemistry-Paraffin: BRD4 Antibody (CL1115) [NBP2-52958] - Staining of human kidney shows moderate nuclear positivity in cells in renal glomeruli and in tubules.
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Applications for BRD4 Antibody (CL1115) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BRD4

BRD4 is encoded by this gene is homologous to the murine protein MCAP, which associates with chromosomes during mitosis, and to the human RING3 protein, a serine/threonine kinase. Each of these proteins contains two bromodomains, a conserved sequence motif which may be involved in chromatin targeting. This gene has been implicated as the chromosome 19 target of translocation t(15;19)(q13;p13.1), which defines an upper respiratory tract carcinoma in young people. Two alternatively spliced transcript variants have been described. [provided by RefSeq]

Long Name

Bromodomain Containing 4

Alternate Names

HUNK1, HUNKI, MCAP

Gene Symbol

BRD4

Additional BRD4 Products

Product Documents for BRD4 Antibody (CL1115) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BRD4 Antibody (CL1115) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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