beta-NGF Antibody (SI79-01)

Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406]

Western Blot: beta-NGF Antibody (SI79-01) [NBP2-67406] - Analysis of NGF on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Hela cell lysateLane 2: HL-60 cell lysate

Immunocytochemistry/ Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406]

Immunocytochemistry/Immunofluorescence: beta-NGF Antibody (SI79-01) [NBP2-67406] - Staining NGF in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406]

Immunohistochemistry-Paraffin: beta-NGF Antibody (SI79-01) [NBP2-67406] - Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-beta-NGF antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-29, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.