Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard (Matched Pair), Neutralization

Label

PE (Excitation = 488 nm, Emission = 575 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 25623

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all beta-NGF Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human beta-NGF
Ser122-Ala241
Accession # CAA36832

Specificity

Detects human beta‑NGF in ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant mouse (rm) beta‑NGF and recombinant rat (rr)  beta-NGF is observed and less than 5% cross-reactivity with recombinant human (rh) BDNF, rhNT‑3, and rhNT‑4 is observed. This antibody neutralizes the bioactivity of rh beta-NGF, rm beta-NGF, and rr beta-NGF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for beta-NGF Antibody (25623) [PE]

Application
Recommended Usage

ELISA Capture (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Detection (Matched Antibody Pair)

Optimal dilutions of this antibody should be experimentally determined.

ELISA Standard (Matched Pair)

Optimal dilutions of this antibody should be experimentally determined.

Neutralization

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: beta-NGF

Nerve growth factor (NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and some sensory neurons, and is composed by 3 types of subunits (alpha, beta and gamma) that specifically interact to form a 7S, 130 kDa molecular weight complex. The beta-NGF subunit is a secreted protein that forms a homodimer before being incorporated into the NGF complex. The human and mouse sequences are very similar and mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5). Deregulation of its expression has been associated with allergic rhinitis.

Long Name

beta Nerve Growth Factor

Alternate Names

betaNGF, NGFB

Gene Symbol

NGF

Additional beta-NGF Products

Product Documents for beta-NGF Antibody (25623) [PE]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for beta-NGF Antibody (25623) [PE]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.