beta-III Tubulin Antibody (TU-20) [FITC]
Novus Biologicals, part of Bio-Techne | Catalog # NB500-620
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Canine
Applications
Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence
Label
FITC (Excitation = 495 nm, Emission = 519 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # TU-20
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions. Rat reactivity reported in scientific literature (PMID: 23984759).
Specificity
The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Marker
Neuronal Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for beta-III Tubulin Antibody (TU-20) [FITC]
Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Immunofluorescence staining of P-19 mouse embryonal carcinoma cell line stimulated to neuronal differentiation by retinoic acid. 2A - Microtubules decorated with neuron-specific anti-betaIII-tubulin (TU-20; red). 2B - Merged image of co-staining with anti-beta-tubulin (TU-06; green). Superposition of red and green colours provided yellow staining. Nuclei were stained with DNA-binding dye (blue).
Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 ug/ml). Nuclei were stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (TU-20) [FITC] [NB500-620] - Staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20; green; 3 ug/ml). Nuclei were stained with DAPI (blue).
Applications for beta-III Tubulin Antibody (TU-20) [FITC]
Application
Recommended Usage
Flow (Intracellular)
1-3 ug/ml
Flow Cytometry
1-3 ug/ml
Immunocytochemistry/ Immunofluorescence
1:400
Application Notes
For Immunohistochemistry-Paraffin: Use standard ABC technique (DAB+). Purity is > 95% (by SDS-PAGE). Western Blot: reducing conditions
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Formulation, Preparation, and Storage
Purification
Size Exclusion Chromatography
Formulation
Phosphate buffered saline (PBS), pH 7.4
Preservative
15mM Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: beta-III Tubulin
Alternate Names
beta-Tubulin III, Beta3-tubulin, betaIII Tubulin, Class III beta-tubulin, TUBB3, Tubulin Beta 3, Tubulin beta-4, Tubulin beta-III
Gene Symbol
TUBB3
Additional beta-III Tubulin Products
Product Documents for beta-III Tubulin Antibody (TU-20) [FITC]
Product Specific Notices for beta-III Tubulin Antibody (TU-20) [FITC]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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