beta-III Tubulin Antibody (SP06-00)

Flow Cytometry: beta-III Tubulin Antibody (SP06-00) [NBP2-67374]

Flow Cytometry: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Flow cytometric analysis of beta-III Tubulin was done on N2A cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/ Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374]

Immunocytochemistry/Immunofluorescence: beta-III Tubulin Antibody (SP06-00) [NBP2-67374] - Staining Tubulin beta-III in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: beta-III Tubulin Antibody (SP06-00) [NBP2-67374]

Western blot analysis on different lysates with NBP2-67374 at 1/20,000 dilution. Lane 1: SH-SY5Y cell lysate (15 µg/Lane) Lane 2: HeLa cell lysate (15 µg/Lane) Lane 3: Neuro-2a cell lysate (15 µg/Lane) Lane 4: NIH/3T3 cell lysate (15 µg/Lane) Lane 5: PC-12 cell lysate (15 µg/Lane) Lane 6: C6 cell lysate (15 µg/Lane) Lane 7: Mouse brain tissue lysate (20 µg/Lane) Lane 8: Rat brain tissue lysate (20 µg/Lane) Predicted band size: 50 kDa Observed band size: 50 kDa Exposure time: 3 minutes 54 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67374 at 1/20,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:50,000 dilution was used for 1 hour at room temperature.