beta-Catenin Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29476

Novus Biologicals, part of Bio-Techne
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NBP2-29476
NBP2-29476-20ug
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all beta-Catenin Antibodies »

Product Specifications

Immunogen

A synthetic peptide from the middle of beta-Catenin protein (Uniprot: P35222)

Reactivity Notes

The amino acid sequence used as the immunogen for this beta Catenin antibody is 100% homologous in a broad range of species.

Localization

Cell surface and cytoplasmic

Marker

Epithelial Cell Marker, Adherens Junction Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

92 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from rabbit anti-serum by Protein A. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-33124)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for beta-Catenin Antibody

Western Blot: beta-Catenin Antibody [NBP2-29476]

Western Blot: beta-Catenin Antibody [NBP2-29476]

Western Blot: beta-Catenin Antibody [NBP2-29476] - Western Blot of beta-Catenin (p120) in human brain 1) absence and 2) presence of immunizing peptide and 3) Mouse brain lysate using beta-Catenin PAb.
Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476]

Immunohistochemistry-Paraffin: beta-Catenin Antibody [NBP2-29476] - Immunohistochemistry-Paraffin: [NBP2-29476] - Formalin-fixed, paraffin-embedded human breast ductal carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific membrane staining in ductal carcinoma.
Flow Cytometry: beta-Catenin Antibody [NBP2-29476]

Flow Cytometry: beta-Catenin Antibody [NBP2-29476]

Flow Cytometry: beta-Catenin Antibody [NBP2-29476] - Flow Cytometric Analysis of PFA fixed HeLa cells using beta-Catenin PAb followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).
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Applications for beta-Catenin Antibody

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunohistochemistry

1-2ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: beta-Catenin

Beta Catenin, an adherens junction protein, was originally identified as a component of cell-cell adhesion structures. It interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Research shows that beta Catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases and phosphatases and Wnt/Wingless. Interplay between these cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta Catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta Catenin has been identified in pilomatrixoma, medulloblastoma and tumor development.

Alternate Names

bCatenin, CTNNB1

Gene Symbol

CTNNB1

UniProt

P35222

Additional beta-Catenin Products

Product Documents for beta-Catenin Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for beta-Catenin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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