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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Avian, Bovine, Chinese Hamster, Fish, Primate, Rabbit

Applications

Block/Neutralize, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Proximity Ligation Assay, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

Product Summary for beta-Actin Antibody

Immunogen

This beta-Actin Antibody was made from a synthetic peptide made to an N-terminal region of human Beta Actin. [UniProt P60709]

Reactivity Notes

Rabbit reactivity and Fish reactivity reported in scientific literature (PMID: 23813946 and 25842206 respectively). Expected to cross-react with a wide range of species due to sequence identity. Bovine reactivity reported in scientific literature (PMID:33066332).

Localization

Cytoplasmic

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta-Actin Antibody

Immunocytochemistry/Immunofluorescence: beta-Actin Antibody [NB600-503] - Cultured pig trabecular meshwork cells stained with beta-Actin antibody at a dilution of 1:500 followed by an anti-rabbit Alexa Fluor488 at 1:1000 dilution (green). Image from verified customer review.
Western Blot: beta-Actin Antibody [NB600-503] - Rabbit polyclonal at 1/5000.
Simple Western: beta-Actin Antibody [NB600-503] - Simple Western lane view shows a specific band for Beta Actin in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Applications for beta-Actin Antibody

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:100

Immunohistochemistry-Paraffin

1:100

Immunoprecipitation

1:10-1:500

Simple Western

1:12.5

Western Blot

1:100-1:2000
Application Notes
Simple Western reported by an internal validation. Separated by Size- Wes/Sally Sue/Peggy Sue, antibody dilution of 1:50. Apparent MW in kDa on Simple Western was 47 kDa; matrix was 12-230.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 4 reviews rated 5 using NB600-503 in the following applications:

Published Applications

Read 122 publications using NB600-503 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: beta-Actin

Actin is a highly conserved protein involved in cell motility, structure, and integrity. Six functional actin isoforms exist and were named based on their electrophoretic mobility and distribution including alpha-cardiac muscle actin (aCAA), alpha-skeletal muscle actin (aSKA), alpha-smooth muscle actin (aSMA), beta-cytoplasmic actin (bCYA), gamma-cytoplasmic actin (gCYA) and gamma-smooth muscle actin (gSMA) (1). The cytoplasmic isoforms, beta-actin and gamma-actin, are nearly identical, differing by only 4 amino acids at the N-terminus with a theoretical molecular weight of 42 kDa (2). Beta-actin is ubiquitously expressed in eukaryotic cells and considered a housekeeping gene which is frequently used as a loading control for assays involving protein detection, such as Western blotting (3,4).

References

1. Vandekerckhove J, Weber K. 1978. At least six different actins are expressed in a higher mammal: an analysis based on the amino acid sequence of the amino-terminal tryptic peptide. J Mol Biol. 126(4):783-802. PMID: 745245

2. Gimona M, Vandekerckhove J, Goethals M, Herzog M, Lando Z, Small JV. (1994) Beta-actin specific monoclonal antibody. Cell Motil Cytoskeleton. 27(2):108-16. PMID: 8162619

3. Holden VI, Lenio S, Kuick R, Ramakrishnan SK, Shah YM, Bachman MA. (2014) Bacterial siderophores that evade or overwhelm Lipocalin 2 induce HIF-1a and pro-inflammatory cytokine secretion in cultured respiratory epithelial cells Infect Immun. 82(9):3826-36 PMID: 24980968

4. Tsai WL, Yeh PH, Tsai CY, Ting CT, Chiu YH, Tao MH, Li WC, Hung SC. (2016) Efficient Programming of Human Mesenchymal Stem Cell Derived Hepatocytes by Epigenetic Regulations. J. Gastroenterol. Hepatol. 32(1):261-269. PMID: 27218433

Alternate Names

ACTB, betaActin

Entrez Gene IDs

60 (Human); 11461 (Mouse); 81822 (Rat)

Gene Symbol

ACTB

UniProt

Product Documents for beta-Actin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta-Actin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for beta-Actin Antibody

Showing  1 - 5 of 6 FAQs Showing All
  • Q: What is the immunogen sequence of this Beta-Actin antibody?

    A: A sequence within the N-terminal region of Human beta Actin. The exact sequence is proprietary.

  • Q: Why is beta actin used as a loading control in blotting ?

    A: Beta actin is a highly-expressed protein found in all cells, and is considered a house keeping protein whose expression is necessary for any cell's proper functioning. For this reason it is used as a loading control to confirm that the same amount of total protein is loaded in each lane of an SDS-PAGE gel so that appropriate comparisons can be made between the actual proteins of interest in different samples.

  • Q: Are the beta-Actin antibodies validated in Simple Western?

    A: Yes, we offer a few beta actin antibodies that have been tested in Simple Western: NBP1-47423, NB600-501, NB600-532, NB600-503, and NB100-56874.

  • Q: Do Beta-Actin antibodies come in lyophilized format?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

  • Q: What is the theoretical molecular weight for Beta-Actin antibodies?

    A: The TMW for beta Actin antibodies is approximately 42 kDa.

  • Q: Do Beta-Actin antibodies come in lyophilized form?

    A: ACTB antibodies like MAB8969 AND MAB8929 come in lyophilized form.

Showing  1 - 5 of 6 FAQs Showing All
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