bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-08580
![bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free Western Blot: bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free [NBP3-08580] -](https://resources.bio-techne.com/images/products/nbp3-08580_mouse-monoclonal-bcl-x-antibody-bcl2l1-1762-azide-and-bsa-free-262025169472.jpeg "Western Blot: bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free [NBP3-08580] -")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # BCL2L1/1762
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Recombinant human bcl-x protein fragment (around aa 1-135) (exact sequence is proprietary) (Uniprot: Q07817)
Localization
Cytoplasmic and cell / nuclear membrane
Specificity
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This monoclonal antibody shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241-amino acid protein which suppresses cell death. And Bcl-XS (short), a 178-amino acid protein lacking a 63-amino acid domain which functions as a dominant inhibitor of Bcl-2.
Marker
Apoptosis Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07416).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free
Western Blot: bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free [NBP3-08580] -
Western Blot Analysis of HeLa, Jurkat, NIH/3T3, Hep G2 and HDLM-2 cell lysates using bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free.Applications for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free
Application
Recommended Usage
ELISA
1-5 ug/ml
Application Notes
ELISA: 1-5ug/ml. Order antibody without BSA. Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Bcl-x
Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
Additional Bcl-x Products
Product Documents for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free
Product Specific Notices for bcl-x Antibody (BCL2L1/1762) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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