bcl-x Antibody (2H12) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11881
Key Product Details
Species Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 2H12
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to amino acids 3 to 14 of the human bcl-xl sequence (Uniprot: Q07817)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Description
Novus Biologicals Mouse bcl-x Antibody (2H12) - BSA Free (NBP3-11881) is a monoclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for bcl-x Antibody (2H12) - BSA Free
![Western Blot: bcl-x Antibody (2H12) [NBP3-11881]](https://resources.bio-techne.com/images/products/bcl-x-Antibody-2H12-Western-Blot-NBP3-11881-img0001.jpg "Western Blot: bcl-x Antibody (2H12) [NBP3-11881]")
Western Blot: bcl-x Antibody (2H12) [NBP3-11881]
Western Blot: bcl-x Antibody (2H12) [NBP3-11881] - Western blot analysis of thymocytes from human, mouse and rat. Cell lysates from approximately 106 cells were loaded into each lane of a 12% SDS-PAGE gel and blotted onto PVDF membranes. Exalpha's anti-Bcl-XL antibody was used at 1 ug/ml - detection was by chemiluminescence detection using an anti-mouse secondary antibody.Applications for bcl-x Antibody (2H12) - BSA Free
Application
Recommended Usage
Western Blot
1 - 5 ug/ml
Application Notes
.
Formulation, Preparation, and Storage
Purification
Ammonium sulfate precipitation
Formulation
PBS
Format
BSA Free
Preservative
0.08% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Bcl-x
Bcl-x is a member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability. Alternative splicing results in two distinct Bcl-x isoforms. Bcl-xL (long) is an anti-apoptotic protein that prevents release of Cytochromec from the mitochondrial intermembrane space into the cytosol. Bcl-xs (short) is a pro-apoptotic member that can initiate programmed cell death.
Long Name
B Cell Lymphoma/Leukemia x
Alternate Names
BCL2L1, Bclx
Gene Symbol
BCL2L1
UniProt
Additional Bcl-x Products
Product Documents for bcl-x Antibody (2H12) - BSA Free
Product Specific Notices for bcl-x Antibody (2H12) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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