Bcl-2 Antibody - BSA Free

Western Blot: Bcl-2 AntibodyBSA Free [NBP2-30108]

Western Blot: Bcl-2 Antibody [NBP2-30108] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines.Loading: 15 ug of lysates per lane.Antibodies: Bcl-2 NBP2-30108, (2 ug/mL), Bcl-2 from Company B, (2 ug/mL), beta-actin (1 ug/mL) and GAPDH (0.02 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] -

Immunocytochemistry/ Immunofluorescence: Bcl-2 Antibody - BSA Free [NBP2-30108] - Figure 7 Immunofluorescence Validation of Bcl-2 in Mouse Brain TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed Mouse Brain Tissue labeling Bcl-2 with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).

Immunohistochemistry-Paraffin: Bcl-2 Antibody - BSA Free [NBP2-30108]

Immunohistochemistry-Paraffin: Bcl-2 Antibody [NBP2-30108] - Immunohistochemistry Validation of Bcl-2 in Human Kidney. Immunohistochemical analysis of paraffin-embedded Human Kidney using anti-Bcl-2 antibody (NBP2-30108) at 2ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.