Bcl-2 Antibody (MSVA-402M) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57315

Recombinant Monoclonal Antibody
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NBP3-57315
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # MSVA-402M

Format

Azide and BSA Free

Concentration

1 mg/ml

View all Bcl-2 Antibodies »

Product Specifications

Immunogen

Recombinant human Bcl-2 fragment Swissprot P10415

Localization

Cytoplasmic

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This antibody is highly specific and optimized for use in Immunohistochemistry.

It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.

Positive Control
Postive control:Tonsil: A strong cytoplasmic bcl-2 staining should be seen in most interfollicular lymphocytes while most germinal centre cells are bcl-2 negative.

Negative Control
Negitve control:Tonsil: The vast majority of lymphocytic cells from germinal centres must be bcl-2 negative while interfollicular lymphocytes are mostly positive.

Applications for Bcl-2 Antibody (MSVA-402M) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bcl-2

The Bcl-2 family of proteins regulate apoptosis by controlling mitochondrial permeability and release of cytochrome c. Bcl-2 is an anti-apoptotic protein that resides in the outer mitochondrial wall and inhibits release of cytochrome c (1). Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies (2). It has also been shown that post-translational modification of Bcl-2, phosphorylation at serine 70, may be required to render its full and/or enhanced anti-apoptotic effect (3, 4).

Long Name

B Cell Lymphoma/Leukemia 2

Alternate Names

Bcl2

Gene Symbol

BCL2

Additional Bcl-2 Products

Product Documents for Bcl-2 Antibody (MSVA-402M) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Bcl-2 Antibody (MSVA-402M) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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