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BCKDK Antibody (HL2710)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48724

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-48724

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2710

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fragment of human BCKDK.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit BCKDK Antibody (HL2710) (NBP3-48724) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for BCKDK Antibody (HL2710)

BCKDK Antibody (HL2710)

Immunohistochemistry-Paraffin: BCKDK Antibody (HL2710) [NBP3-48724] -

BCKDK antibody [HL2710] detects BCKDK protein at mitochondria by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
BCKDK stained by BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
BCKDK Antibody (HL2710)

Western Blot: BCKDK Antibody (HL2710) [NBP3-48724] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
BCKDK Antibody (HL2710)

Western Blot: BCKDK Antibody (HL2710) [NBP3-48724] -

Wild-type (WT) and BCKDK knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with BCKDK antibody [HL2710] (NBP3-48724) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for BCKDK Antibody (HL2710)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BCKDK

BCKDK catalyzes the phosphorylation of DNA at 5'-hydroxyl termini and can dephosphorylate its 3'-phosphate termini. Plays an important function in DNA repair following ionizing radiation or oxidative damage

Alternate Names

BCKDHKIN, branched chain alpha-ketoacid dehydrogenase kinase, branched chain ketoacid dehydrogenase kinase, EC 2.7.11, EC 2.7.11.4, mitochondrial

Gene Symbol

BCKDK

Additional BCKDK Products

Product Documents for BCKDK Antibody (HL2710)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BCKDK Antibody (HL2710)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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