Bassoon Antibody (L124/59) - Chimeric - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52352

Recombinant Monoclonal Antibody
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NBP3-52352-25ul
NBP3-52352-100ul
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Chicken IgY Clone # L124/59

Format

BSA Free

Concentration

1 mg/ml

View all Bassoon Antibodies »

Product Specifications

Immunogen

Fusion protein amino acids 157-517 (zinc finger domains) of rat Bassoon (Uniprot#: O88778) produced recombinantly in E. Coli

Clonality

Monoclonal

Host

Chicken

Isotype

IgY

Description

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L124/59 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L124/59 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in HEK293 cells and then affinity purified from the cell culture media.

Scientific Data Images for Bassoon Antibody (L124/59) - Chimeric - BSA Free

Immunohistochemistry-Paraffin: Bassoon Antibody (L124/59) - Chimeric - BSA Free [NBP3-52352]

Immunohistochemistry-Paraffin: Bassoon Antibody (L124/59) - Chimeric - BSA Free [NBP3-52352]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with NBP3-52352 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 100X.
Immunohistochemistry-Paraffin: Bassoon Antibody (L124/59) - Chimeric - BSA Free [NBP3-52352]

Immunohistochemistry-Paraffin: Bassoon Antibody (L124/59) - Chimeric - BSA Free [NBP3-52352]

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum region stained with NBP3-52352 at 1:100 dilution. DAPI nuclear stain (blue) shows cell nuclei. This antibody stains the presynaptic sites on Purkinje cell dendrites in the molecular layer of the cerebellum. Magnification 400X.

Applications for Bassoon Antibody (L124/59) - Chimeric - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry-Paraffin

1:100

Western Blot

1:500-1:1000
Application Notes
400 kDa for full-length protein, many smaller bands possible depending on sample used

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

1X PBS (pH 7.4)

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Bassoon

Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96% (1). The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains (1). The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration (1,3). Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors (2). Another presynaptic protein, Piccolo (4) was found to colocalize with Bassoon in cultured hippocampal neurons (1). These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release (1).

Alternate Names

bassoon (presynaptic cytomatrix protein)

Gene Symbol

BSN

Additional Bassoon Products

Product Documents for Bassoon Antibody (L124/59) - Chimeric - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Bassoon Antibody (L124/59) - Chimeric - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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