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BAK Antibody, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NB100-893

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NB100-893

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Summary for BAK Antibody

Immunogen

Peptide with sequence ASGQGPGPPRQE-C corresponding to N-Terminus according to NP_001179.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for BAK Antibody

Western Blot: BAK Antibody [NB100-893] - Staining of Human Adrenal Gland lysate (35 ug protein in RIPA buffer). BAK antibody at 2 ug/mL. Detected by chemiluminescence.

Applications for BAK Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.5 - 1 ug/mL
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: BAK

Apoptosis or programmed cell death is a physiological cellular process characterized by cell shrinkage, membrane blebbing, DNA fragmentation, and release of Cytochrome C from the mitochondria. It is utilized by the organism to get rid of unwanted cells, which is critical for normal development and homeostasis of an organism. Disregulation of normal apoptosis process have been implicated in a variety of diseases, including cancer, autoimmune diseases, viral infections, etc. Programmed cell death occurs through complex cascades of cell signaling in which Bcl2 family members, among others, play an important role.The Bcl2 family of proteins regulate apoptosis as well as execute death signals at the mitochondrion. Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of holologous proteins which function in the regulation of apoptosis. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relativeratios of the different Bcl2 family proteins. Bak is a 211 aa protein (24kD) which has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. Bak has been localized by immunohistochemistry to the cytosol of cells and stains in a punctate pattern characteristic of other bcl2 family proteins, which have been localized to intracellular organelles. Immunohistochemical studies further show that Bak expression is widespread, and may show some correlation with the apoptopic state of cells.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Entrez Gene IDs

578 (Human)

Gene Symbol

BAK1

Additional BAK Products

Product Documents for BAK Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAK Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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