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BAK Antibody (38E2) - BSA Free, Novus Biologicals

Bio-Techne includes Novus Biologicals | Catalog # NBP1-74026

Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 38E2

Format

BSA Free

Concentration

1 mg/ml

Product Summary for BAK Antibody (38E2) - BSA Free

Immunogen

Recombinant human BAK (29-187aa) purified from E.coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for BAK Antibody (38E2) - BSA Free

Western Blot: BAK Antibody (AT38E2) [NBP1-74026] - The cell lysate (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human BAK1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : A549 cell lysateLane 5 : Jurkat cell lysateLane 6 : MCF7 cell lysateLane 7 : PC3 cell lysate
Immunocytochemistry/Immunofluorescence: BAK Antibody (AT38E2) [NBP1-74026] - Analysis of BAK1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human HeLa antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green)
Flow Cytometry: BAK Antibody (AT38E2) [NBP1-74026] - Flow Cytometry: BAK Antibody (8B4) [PE] [NBP1-74026PE] - An intracellular stain was performed on Jurkat cells with BAK antibody (8B4) NBP1-74026PE (blue) and a matched isotype control NB600-985PE (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE form of this antibody.

Applications for BAK Antibody (38E2) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:250-500

Western Blot

1:250-500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 2 publications using NBP1-74026 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: BAK

BAK1 encodes an receptor-like kinase (RLK) with a putative extracellular domain, a single transmembrane domain, an intracellular-juxtamembrane domain, and a kinase domain. BAK1 expression is associated with the progression of Prostate cancer (CaP). BAK1 appears to function in distinct receptor-signaling complexes to integrate multiple microbe-associated molecular pattern (MAMP) perception into downstream-signaling events. BAK1 can serve multiple purposes to simultaneously modulate MAMP-receptor complexes, BR signaling, and cell death for the benefit of bacterial infection, life style, and fitness.

Long Name

Bcl-2 Antagonist/Killer

Alternate Names

BAK1, BCL2L7

Gene Symbol

BAK1

Additional BAK Products

Product Documents for BAK Antibody (38E2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BAK Antibody (38E2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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