BAG3 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-15543

Novus Biologicals, part of Bio-Techne
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NBP2-15543
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (94%), Rat (95%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all BAG3 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BAG3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for BAG3 Antibody - BSA Free

Western Blot: BAG3 Antibody [NBP2-15543]

Western Blot: BAG3 Antibody [NBP2-15543]

Western Blot: BAG3 Antibody [NBP2-15543] - BAG3 antibody detects BAG3 protein by Western blot analysis. A. 50 ug mouse heart lysate/extract 7. 5 % SDS-PAGEBAG3 antibody dilution: 1:1000.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - BAG3 in high-grade serous carcinoma. 1:100 dilution, pH=6 retrieval buffer, Dako FLEX+ protocol. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543]

Immunohistochemistry-Paraffin: BAG3 Antibody [NBP2-15543] - Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using antibody at 1:100 dilution.
View 1 more images

Applications for BAG3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 3 using NBP2-15543 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: BAG3

BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The protein encoded by this gene contains a WW domain in the N-terminal region and a BAG domain in the C-terminal region. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner. [provided by RefSeq]

Alternate Names

BAG family molecular chaperone regulator 3, BAG-3, BAG-family molecular chaperone regulator-3, Bcl-2-associated athanogene 3, BCL2-associated athanogene 3, BCL2-binding athanogene 3, Bcl-2-binding protein Bis, BIS, CAIR-1, Docking protein CAIR-1, MGC104307

Gene Symbol

BAG3

UniProt

O95817 (Human)

Additional BAG3 Products

Product Documents for BAG3 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for BAG3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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