B-Raf [p Ser445] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05377

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NBP3-05377-100ul
NBP3-05377-20ul
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all B-Raf Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S445 of human BRAF (NP_004324.2). RDSSD

Modification

p Ser445

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for B-Raf [p Ser445] Antibody

Western Blot: B-Raf [p Ser445] AntibodyBSA Free [NBP3-05377]

Western Blot: B-Raf [p Ser445] AntibodyBSA Free [NBP3-05377]

Western Blot: B-Raf [p Ser445] Antibody [NBP3-05377] - Western blot analysis of extracts of Raji cells, using B-Raf pAb (NBP3-05377) at 1:2000 dilution or BRAF antibody (A0038).Raji cells were treated by PMA/TPA (200nM) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 10s.

Applications for B-Raf [p Ser445] Antibody

Application
Recommended Usage

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: B-Raf

B-Raf, also know as c-Rmil, is a Serine/Threonine protein kinase member of the Raf family which includes Raf-1 and A-Raf. B-Raf is involved in the transduction of mitogenic signals from the cell membrane to the nucleus (1). Composed of three conserved region (CR1, CR2, CR3) B-Raf is expressed primarily in the brain and in the nervous system (2). It has been observed that MAPK is activated by B-Raf in response to nerve growth factor (3). More than 60% of malignant melanomas were found to contain a specific mutation, B-Raf (V599E) the product of which possesses constitutive kinase activity (4). Mutations in B-Raf have also been identified in lung cancer and non-Hodgkin lymphoma.

Long Name

v-Raf Murine Sarcoma Viral Oncogene Homolog B1

Alternate Names

BRaf, BRAF1, RAFB1

Gene Symbol

BRAF

Additional B-Raf Products

Product Documents for B-Raf [p Ser445] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for B-Raf [p Ser445] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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