ATG4B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24709

Novus Biologicals, part of Bio-Techne
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NBP2-24709SS
NBP2-24709
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Canine (100%), Chimpanzee (100%), Monkey (100%), Opossum (94%), Orangutan (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all ATG4B Antibodies »

Product Specifications

Immunogen

A portion of amino acid 350-400 of human APG4B was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 88% in Zebrafish and Porcine.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit ATG4B Antibody - BSA Free (NBP2-24709) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ATG4B Antibody - BSA Free

Western Blot: ATG4B Antibody [NBP2-24709]

Western Blot: ATG4B Antibody [NBP2-24709]

Western Blot: ATG4B Antibody [NBP2-24709] - Analysis of APG4B in HeLa cell lysate in the 1) absence, and 2) presence of immunizing peptide and 3) RAW cell lysate using NBP2-24709 at 2 ug/ml.
Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709]

Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709]

Immunohistochemistry-Paraffin: ATG4B Antibody [NBP2-24709] - Analysis of human heart using NBP2-24709 at 10 ug/ml.

Applications for ATG4B Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

10 ug/ml

Western Blot

1-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATG4B

FUNCTION: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. ENZYME REGULATION: Inhibited by N-ethylmaleimide. SUBCELLULAR LOCATION: Cytoplasm (Probable). ALTERNATIVE PRODUCTS: 5 named isoforms produced by alternative splicing.

Long Name

ATG4 Autophagy Related 4 Homolog B

Alternate Names

Apg4B, AUTL1

Gene Symbol

ATG4B

Additional ATG4B Products

Product Documents for ATG4B Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ATG4B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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