ATF6 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77251
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA
Cited:
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6. The immunogen is located within amino acids 30 - 80 of ATF6.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ATF6 Antibody - BSA Free
Immunohistochemistry: ATF6 Antibody - BSA Free [NBP1-77251] -
Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-ATF6 antibody at 5 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -
Loading: 15 ug of rat kidney lysate Antibodies: ATF6, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot: ATF6 Antibody - BSA Free [NBP1-77251] -
Loading: 15 ug of mouse tissue lysate Antibodies: ATF6, 1 ug/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Applications for ATF6 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
5 ug/mL
Western Blot
0.5-1 ug/ml
Application Notes
Use in IHC-P reported in scientific literature (PMID:33603856).
Formulation, Preparation, and Storage
Purification
Peptide affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ATF6
Long Name
Activating Transcription Factor 6
Alternate Names
ACHM7, ATF6 alpha
Gene Symbol
ATF6
UniProt
Additional ATF6 Products
Product Documents for ATF6 Antibody - BSA Free
Product Specific Notices for ATF6 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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