ATAD2 Antibody (CL0182) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44437

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0182

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ATAD2 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q6PL18, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for ATAD2 Antibody (CL0182) - Azide and BSA Free

ATAD2 Antibody (CL0182) - Azide and BSA Free Western Blot: ATAD2 Antibody [NBP3-44437]

Western Blot: ATAD2 Antibody [NBP3-44437]

Lane 1: Marker [kDa]
Lane 2: ATAD2 Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag in mammalian HEK293T cells)
Lane 3: Negative control (vector only transfected HEK293T lysate)
Lane 4: U-251
ATAD2 Antibody (CL0182) - Azide and BSA Free Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP3-44437]

Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP3-44437]

Analysis in human testis and skeletal muscle tissues using NBP3-44437 antibody. Corresponding ATAD2 RNA-seq data are presented for the same tissues.
ATAD2 Antibody (CL0182) - Azide and BSA Free Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP3-44437]

Immunohistochemistry-Paraffin: ATAD2 Antibody [NBP3-44437]

Staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.
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Applications for ATAD2 Antibody (CL0182) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue. Immunocytochemistry/ Immunofluorescence/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ATAD2

ATAD2 is a large family of ATPases has been described, whose key feature is that they share a conserved region of about 220 amino acids that contains an ATP-binding site. The proteins that belong to this family either contain one or two AAA (ATPases Associated with diverse cellular Activities) domains. AAA family proteins often perform chaperone-like functions that assist in the assembly, operation, or disassembly of protein complexes. The protein encoded by this gene contains two AAA domains, as well as a bromodomain. [provided by RefSeq]

Long Name

ATPase family, AAA domain containing 2

Alternate Names

ANCCA, CT137, PRO2000

Gene Symbol

ATAD2

Additional ATAD2 Products

Product Documents for ATAD2 Antibody (CL0182) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ATAD2 Antibody (CL0182) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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