ASTE1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-81661
![Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]](https://resources.bio-techne.com/images/products/ASTE1-Antibody-Immunohistochemistry-Paraffin-NBP1-81661-img0007.jpg "Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]")
![Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]](https://resources.bio-techne.com/images/products/ASTE1-Antibody-Immunohistochemistry-Paraffin-NBP1-81661-img0008.jpg "Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]")
![Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]](https://resources.bio-techne.com/images/products/ASTE1-Antibody-Immunohistochemistry-Paraffin-NBP1-81661-img0009.jpg "Immunohistochemistry-Paraffin: ASTE1 Antibody [NBP1-81661]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: CFVQCFSEADRDIMTLANHWNCPVLSSDSDFCIFDLKTGFCPLNSFQWRNMNTIKGTQNYIPAKCFSLDAFCHHFSNMNKALLPLFAVLCGNDHVNLPIME
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ASTE1 Antibody - BSA Free (NBP1-81661) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-ASTE1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ASTE1 Antibody - BSA Free
![Western Blot: ASTE1 Antibody [NBP1-81661]](https://resources.bio-techne.com/images/products/ASTE1-Antibody-Western-Blot-NBP1-81661-img0010.jpg "Western Blot: ASTE1 Antibody [NBP1-81661]")
Western Blot: ASTE1 Antibody [NBP1-81661]
Western Blot: ASTE1 Antibody [NBP1-81661] - Analysis in control (vector only transfected HEK293T lysate) and ASTE1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]](https://resources.bio-techne.com/images/products/ASTE1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-81661-img0006.jpg "Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]")
Immunocytochemistry/ Immunofluorescence: ASTE1 Antibody [NBP1-81661]
Immunocytochemistry/Immunofluorescence: ASTE1 Antibody [NBP1-81661] - Staining of human cell line U-2 OS shows localization to endoplasmic reticulum. Antibody staining is shown in green.Applications for ASTE1 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ASTE1
Alternate Names
asteroid homolog 1 (Drosophila), HT001, MGC129980, protein asteroid homolog 1
Gene Symbol
ASTE1
Additional ASTE1 Products
Product Documents for ASTE1 Antibody - BSA Free
Product Specific Notices for ASTE1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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