ASCL4 Antibody - BSA Free

Immunohistochemistry-Paraffin: ASCL4 Antibody - Azide and BSA Free [NBP2-92741]

Immunohistochemistry-Paraffin: ASCL4 Antibody [NBP2-92741] - Human breast cancer using ASCL4 antibody at dilution of 1:100 (40x lens).

Western Blot: ASCL4 Antibody - BSA Free [NBP2-92741] -

Inhibition of TRIM72 partially reverses the inhibitory effect of MLL1 inhibition on ferroptosis of HTR-8/SVneo cells. HTR-8/SVneo cells were transfected with sh-TRIM72, with sh-NC as the control. A: Detection of transfection efficiency by qRT-PCR; then HTR-8/SVneo cells were treated with sh-MLL1 for a combined experiment. B: Detection of TRIM72, ASCL4, GPX4, and FTH1 expressions in cells by Western blot. C: Detection of cell viability by CCK-8 assay. D-F: Detection of MDA, Fe2+, and GSH levels in cells by kits. G: Detection of ROS levels in cells by fluorescence. H: Detection of cell invasion and migration by Transwell. The cell experiments were repeated three times independently. Data in panel A were analyzed by t test. Data in panels CDEFGH were analyzed by one-way ANOVA, and data in panel B were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://biologydirect.biomedcentral.com/articles/10.1186/s13062-024-005…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASCL4 Antibody - BSA Free [NBP2-92741] -

Overexpression of RBM15 partially reverses the inhibitory effect of MLL1 inhibition on ferroptosis of HTR-8/SVneo cells. HTR-8/SVneo cells were transfected with oe-RBM15, with oe-NC as the control. A: Detection of transfection efficiency by qRT-PCR; then HTR-8/SVneo cells were treated with sh-MLL1 for a combined experiment. B: Detection of RBM15, TRIM72, ASCL4, GPX4, and FTH1 in cells by Western blot. C: Detection of cell viability by CCK-8 assay. D-F: Detection of MDA, Fe2+, and GSH levels in cells by kits. G: Detection of ROS levels in cells by fluorescence. H: Detection of cell invasion and migration by Transwell. The cell experiments were repeated three times independently. Data in panel A were analyzed by t test. Data in panels CDEFGH were analyzed by one-way ANOVA, and data in panel B were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://biologydirect.biomedcentral.com/articles/10.1186/s13062-024-005…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASCL4 Antibody - BSA Free [NBP2-92741] -

Overexpression of ADAM9 partially reverses the inhibitory effect of MLL1 inhibition on ferroptosis of HTR-8/SVneo cells. HTR-8/SVneo cells were transfected with oe-ADAM9, with oe-NC as the control. A: Detection of transfection efficiency by qRT-PCR; then HTR-8/SVneo cells were treated with sh-MLL1 for a combined experiment. B: Detection of ADAM9, ASCL4, GPX4, and FTH1 expressions in cells by Western blot. C: Detection of cell viability by CCK-8 assay. D-F: Detection of MDA, Fe2+, and GSH levels in cells by kits. G: Detection of ROS levels in cells by fluorescence. H: Detection of cell invasion and migration by Transwell. The cell experiments were repeated three times independently. Data in panel A were analyzed by t test. Data in panels CDEFGH were analyzed by one-way ANOVA, and data in panel B were analyzed by two-way ANOVA, followed by Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01 Image collected and cropped by CiteAb from the following open publication (https://biologydirect.biomedcentral.com/articles/10.1186/s13062-024-005…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.