ASC/TMS1 Antibody - BSA Free

Western Blot: ASC/TMS1 AntibodyBSA Free [NBP1-78977]

Western Blot: ASC/TMS1 Antibody [NBP1-78977] - Whole cell protein from (1) THP1 and (2) HL60 was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 4.0 ug/ml anti-ACS/TMS1 in 1% milk, and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Flow Cytometry: ASC/TMS1 Antibody - BSA Free [NBP1-78977]

Flow Cytometry: ASC/TMS1 Antibody - BSA Free [NBP1-78977] - An intracellular stain was performed on MCF7 cells with ASC/TMS1 NBP1-78977 (blue) and a matched isotype control NBP2-24891 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (SA5-10033, Thermo Fisher).

Immunocytochemistry/ Immunofluorescence: ASC/TMS1 Antibody - BSA Free [NBP1-78977]

Immunocytochemistry/Immunofluorescence: ASC/TMS1 Antibody [NBP1-78977] - Tested in MCF-7 cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Immunohistochemistry: ASC/TMS1 Antibody - BSA Free [NBP1-78977]

Immunohistochemistry: ASC/TMS1 Antibody [NBP1-78977] - Tested in mouse intestine at a 1:400 dilution.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Nicorandil alleviates cardiac apoptosis in type 2 diabetic rat. A: TUNEL staining and TUNEL‐positive cells rate. B: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3. C: Level of nitric oxide and ADMA in serum. D: Western blot analysis of p‐eNOS. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. *P < 0.05 compared with control; #P < 0.05 compared with DM; #P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

PI3K/AKT pathway inhibition blocked the protection of nicorandil on H9c2 cardiomyocyte treated with high glucose. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors (scale bar: 20 um). C: Western blot analysis of p‐eNOS in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. N: Nicorandil (100 umol); MTF: miltefosine (100 umol); Rapa: rapamycin (100 umol) NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). *P < 0.05 compared with NG; #P < 0.05 compared with HG; &P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Nicorandil protects H9C2 cells from apoptosis through PI3K/AKT pathway. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and 5‐HD which is a inhibitor of nicorandil. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor（5‐HD, 500 umol) (scale bar: 20 um). I:NG, II:HG, III:HG + N, IV:HG + N+5‐HD; C: Western blot analysis of phosphorylation level of PI3K, AKT, eNOS and mTOR in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor（5‐HD). N: Nicorandil (100 umol); NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). *P < 0.05 compared with NG; #P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Effects of abrocitinib on NF kappaB-related inflammation and pyroptosis pathways. On the 1st day after TBI, NF kappaB-related inflammation and the activation of pyroptosis pathways were dramatically increased. After abrocitinib treatment, the indicators of NF kappaB-related inflammation and pyroptosis pathways were significantly decreased, as can be seen from the WB (n = 5–9 one-way ANOVA with Tukey’s post-hoc test) and GSDMD IHC staining (A–C) (n = 6 Kruskal-Wallis test). From the ELISA results, the changes in inflammatory cytokines (IL-1 beta and IL-18) after brain injury and the effects of abrocitinib were precisely revealed (D,E) (n = 5–6 one-way ANOVA with Tukey’s post-hoc test). All data are shown as mean +/- SD. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36429017), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Apoptosis level reduced after nicorandil treatment in high glucose‐induced H9c2 cardiomyocyte. A: Western blot analysis of bax and bcl‐2 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment with different concentrations for 24 h. B: Western bolt analysis of cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment. HG (33.3 mmol/L), NG (5.5 mmol/L), n1: nicorandil (10 umol); n2: nicorandil (50 umol); n3: nicorandil (100 umol). #P < 0.05 compared with NG; *P < 0.05 compared with HG, Data are means +/- SD Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31131539), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Effects of Mito-Vit-E and TLR9 inhibitor OND-I in LPS-challenged cardiomyocytes.Cultured neonatal cardiomyocytes from rats were treated with +/-LPS (100 ng/ml), +/-Mito-Vit-E (MVE) (1μM), or +/-ODN-I (0.5 μM) 4 hours prior to harvesting. A. Mitochondrial superoxide was labeled with MitoSox Red and quantified by flow cytometry. B. Mitochondrial biogenesis was quantified in live cells using MitoBiogenesis In-Cell ELISA assay. C. Levels of mtDNA in cell medium and in cytoplasm were measured by real-time PCR. D. Cells apoptosis was evaluated by TUNEL assay (green). Cell nucleuses were identified by DAPI staining (blue). E. Expression of MyD88, RAGE, ASC and activated form of caspase 1 were determined in cell lysates by western blot using GAPDH as a loading control, and results were quantified by densitometry. F. Cellular production of IL–1 beta was measured by ELISA. All the measurements were normalized by cell numbers and obtained in triplicate. All values are means +/-SE. Significant differences are shown as * between control and LPS and delta between vehicle and drug-treated groups (p<0.02 for A-C and p<0.01 for E-F, n = 4). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/26448624), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Analysis of NLRP3, NLRC4, NAIP, AIM2, ASC, Caspase-4, Caspase-1, beta-actin and GAPDH protein expression by immunoblotting. (A) Represents the immunoblotting results of NLRP3, NLRC4, NAIP, Caspase-1, GAPDH, AIM2, ASC, Caspase-4 and beta-actinin UPEC infected UTI patients and controls group. Scattered plots showing individual densitometric values (IDV) of NLRP3 (B), NLRC4 (C), NAIP (D), AIM2 (E), ASC (F), Caspase-4 (G), and Caspase-1 (H). Results were expressed as average densitometric ratio in patients and controls group +/- SD. P-value is ∗∗∗p = 0.0001 and n.s, non-significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

QWZK inhibited NLRP3 inflammasome activation in ALI rats induced by LPS. (A) Western blot assay of NLRP3, pro-caspase-1, cleaved caspase-1, and ASC in different groups. (B–D) The protein expression was analyzed by gray scale. Data were presented as the mean +/- SEM, n = 8. #p < 0.05 vs. control group, ##p < 0.01 vs. control group, ###p < 0.001 vs. control group. *p < 0.05 vs. LPS group, **p < 0.01 vs. LPS group, ***p < 0.001 vs. LPS group. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fphar.2021.790072/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

Inhibition of FOXO1 or autophagy compromised the antioxidative and anti‐inflammatory actions of AVE in mice. (a, b) The selective FOXO1 inhibitor, AS, and the autophagy inhibitor, CQ, abrogated the immune‐regulatory effect of AVE on microglial polarization. (c) AS and CQ both inhibited AVE‐induced alleviation of microglial activation (IBA‐1 staining) and ROS generation (DHE staining) following LPS exposure. (d, e) Representative western blots (d) and statistical graphs (e) of the major components of NLRP3 inflammasomes. Scale bar = 50 μm. Data are means +/- SD (n = 6–7). *p < 0.05, **p < 0.01 compared to control group. +p < 0.05, ++p < 0.01 compared to LPS group. #p < 0.05, ##p < 0.01 compared to LPS + AVE group Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34529881), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ASC/TMS1 Antibody - BSA Free [NBP1-78977] -

The measurement of pyroptosis-related markers after intervention in the mice. (A) IF staining (×200) (left) and the IOD of NLRP3 in GAS of db/db mice (n = 6). The protein expression levels of (B) NLRP3; (C) ASC; (D) Caspase-1 and (E) GSDMD were detected by western blot in the GAS of db/db mice (n = 6). Serum inflammatory markers (F) IL-1 beta and (G) IL-18 by ELISA (n = 8). (#) Significant difference compared with D group; (&) significant difference compared with DI group; ($) significant difference compared with DE group (p < 0.05). Image collected and cropped by CiteAb from the following open publication (https://www.mdpi.com/1420-3049/29/3/712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.