ARID5B Antibody - BSA Free

Western Blot: ARID5B Antibody - BSA Free [NBP1-83622] -

Trimethylation of histone 3 lysine 4 (H3K4me3)‐mediated ARID5B expression at its promoter. (A) Workflow of beta2GPI/anti‐ beta2GPI immune complex (IC) treatment and OICR‐9424 exposure in an ex vivo monocyte or THP‐1 cell model that partially mimicked antiphospholipid syndrome (APS). (B) Heatmaps of H3K4me3 Cleavage Under Targets and Tagmentation (CUT&Tag) in an ex vivo THP‐1 cell and monocyte model of APS. (C) Heatmaps of Transposase‐Accessible Chromatin using sequencing (ATAC‐Seq) in an ex vivo THP‐1 cell and monocyte model of APS. (D) Venn diagram showed the intersection of the unique peaks (ARID5B) in the IC group compared to that in the negative control (NC) group between H3K4me3 CUT&Tag and ATAC‐Seq. (E) Integrative Genomics Viewer (IGV) and (F) quantitative PCR (qPCR) showed the relative enrichment levels of H3K4me3 at the promoter of ARID5B. (G) Chromatin accessibility at the ARID5B promoter was displayed using IGV. (H) The protein levels of ARID5B in the ex vivo model of APS were detected using western blotting. (I) Real‐time quantitative PCR (RT‐qPCR) determined the mRNA expression of ARID5B in the ex vivo model of APS. Data information: error bars represent the mean +/- SD of at least three independent experiments. **p < .01; ***p < .001. beta2GPI, beta2‐glycoprotein I. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38224186), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ARID5B Antibody - BSA Free [NBP1-83622] -

ARID5B transcriptionally activated LINC01128 expression. (A) Schematic representation of eight target long non‐coding RNAs (lncRNAs) of ARID5B in anti‐ARID5B Cleavage Under Targets and Tagmentation (CUT&Tag) in THP‐1 cells. (B) The two binding sites of ARID5B at the LINC01128 promoter were predicted using http://bioinfo.life.hust.edu.cn/hTFtarget#!/website. (C) Western blotting and (D) real‐time quantitative PCR (RT‐qPCR) analysis of ARID5B expression after transfection with two shRNAs (shARID5B#1 and shARID5B#2) or overexpression lentivirus in THP‐1 cells and monocytes. (E) RT‐qPCR analysis of LINC01128 expression after transfection with the above shRNAs or overexpression lentivirus. (F) RT‐qPCR analysis of LINC01128 expression after treatment with beta2GPI/anti‐ beta2GPI immune complex (IC) in shARID5B‐THP‐1 cells. (G) Integrative Genomics Viewer (IGV) and quantitative PCR (qPCR) representation of anti‐ARID5B CUT&Tag at the LINC01128 promoter in the scrambled negative control (shNC)‐ and shARID5B‐THP‐1 cells. (H) Schematic representation of the mutated sequences of potential ARID5B‐binding sites on the LINC01128 promoter; luciferase activity after transfection with a reporter containing wild‐type (WT‐LINC01128) or mutant LINC01128 (Mut‐LINC01128) promoter constructs in 293T cells. (I) RNA fluorescence in situ hybridisation (FISH) assay showing the subcellular localisation of LINC01128 in THP‐1 cells; U6 was used as a nuclear localisation control; green, LINC01128; red, U6; blue, DAPI. (J) Nuclear fractionation and RT‐qPCR analysis of LINC01128 expression in the nucleus and cytoplasm. Data information: error bars represent the mean +/- SD of at least three independent experiments. ns, not significant; *p < .05; **p < .01; ***p < .001. beta2GPI, beta2‐glycoprotein I. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38224186), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: ARID5B Antibody - BSA Free [NBP1-83622] -

ARID5B suppresses the expression of CCR2, MCP-1, and TNF-alpha and the migration and adhesion of THP-1 cells. A The protein and mRNA expression of ARID5B in wild-type, ARID5B-overexpressing and ARID5B-knockout THP-1 cells. B Relative mRNA expression levels of CCR2, MCP-1, and TNF-alpha in THP-1 cells. C The migration capacity of THP-1 cells was assessed by Transwell migration assays with an inverted microscope (magnification × 100). D The adhesion of THP-1 cells to HUVECs was assessed using a fluorescence microscope (magnification × 100). All plotted values are the mean +/- SE values of at least three independent experiments. WT, wild type; OE, overexpression; KO, knockout. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35227297), licensed under a CC-BY license. Not internally tested by Novus Biologicals.