Aquaporin-4 Antibody (AQP4/3323)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44612
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Flow Cytometry
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # AQP4/3323
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Human recombinant Aquaporin-4 protein fragment (around aa200-323) (Uniprot: P55087). Exact sequence is proprietary.
Localization
Basolateral cell membrane, Cell membrane, Cell projection, Endosome membrane, Sarcolemma
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.
Scientific Data Images for Aquaporin-4 Antibody (AQP4/3323)
Western Blot: Aquaporin-4 Antibody (AQP4/3323) [NBP3-44612] -
Western Blot Analysis of HepG2 cell lysate using Aquaporin-4 Antibody (AQP4/3323).
Flow Cytometry: Aquaporin-4 Antibody (AQP4/3323) [NBP3-44612] -
Flow Cytometric Analysis of PFA-fixed Ramos cells using Aquaporin-4 Antibody (AQP4/3323). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Array: Aquaporin-4 Antibody (AQP4/3323) [NBP3-44612] -
Analysis of Protein Array containing more than 19,000 full-length human proteins using Aquaporin-4 Antibody (AQP4/3323).Applications for Aquaporin-4 Antibody (AQP4/3323)
Application
Recommended Usage
Flow Cytometry
1-2ug/million cells
Immunohistochemistry
1-2ug/ml
Western Blot
2-4ug/ml
Application Notes
Immunohistochemistry: 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Aquaporin 4/AQP4
Long Name
Aquaporin 4
Alternate Names
AQP-4, AQP4, MIWC, WCH4
Gene Symbol
AQP4
Additional Aquaporin 4/AQP4 Products
Product Documents for Aquaporin-4 Antibody (AQP4/3323)
Product Specific Notices for Aquaporin-4 Antibody (AQP4/3323)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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