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Anillin Antibody (CL0303) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-43945

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0303

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q9NQW6, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Anillin Antibody (CL0303) - Azide and BSA Free (NBP2-52908) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Anillin Antibody (CL0303) - Azide and BSA Free

Anillin Antibody (CL0303) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP3-43945]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP3-43945]

Staining in A431 cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).
Anillin Antibody (CL0303) - Azide and BSA Free Immunohistochemistry-Paraffin: Anillin Antibody [NBP3-43945]

Immunohistochemistry-Paraffin: Anillin Antibody [NBP3-43945]

Analysis in human testis and skeletal muscle tissues using NBP3-43945 antibody. Corresponding Anillin RNA-seq data are presented for the same tissues.
Anillin Antibody (CL0303) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP3-43945]

Immunocytochemistry/ Immunofluorescence: Anillin Antibody [NBP3-43945]

Staining in U251 cell line with Anti-Anillin monoclonal antibody) staining in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Applications for Anillin Antibody (CL0303) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Anillin

Anillin is a 124 kDa scaffolding protein that plays an important role in cell division. Anillin interacts with septins and mediates septin filament assembly and stabilization in the construction of the contractile ring responsible for cleavage furrow formation and cytokinesis.

Alternate Names

actin-binding protein anillin, ANILLIN, anillin (Drosophila Scraps homolog), actin binding protein, anillin, actin binding protein, anillin, actin binding protein (scraps homolog, Drosophila), DKFZp779A055, Drosophila Scraps homolog, scra, Scraps

Gene Symbol

ANLN

Additional Anillin Products

Product Documents for Anillin Antibody (CL0303) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Anillin Antibody (CL0303) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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