Amyloid Fibrils Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97929
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot
Cited:
Immunohistochemistry-Paraffin, Western Blot, Cytometric Bead Assay Standard
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Fibrils prepared from human amyloid beta 42 peptide
Localization
Membrane
Specificity
Recognizes generic epitopes common to many amyloid fibrils and fibrillar oligomers, but not prefibrillar oligomers or natively folded proteins. Expected to detect in Mouse and Rat based on species homology.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Amyloid Fibrils Antibody - BSA Free
![Western Blot: Amyloid Fibrils Antibody [NBP1-97929]](https://resources.bio-techne.com/images/products/Amyloid-Fibrils-Antibody-Western-Blot-NBP1-97929-img0008.jpg "Western Blot: Amyloid Fibrils Antibody [NBP1-97929]")
Western Blot: Amyloid Fibrils Antibody [NBP1-97929]
Western Blot: Amyloid Fibrils Antibody [NBP1-97929] - Western blot analysis of Human Abeta42 fibrils and prefibrillar oligomers showing detection of Amyloid Fibrils Fibrils (OC) protein using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:1000. Courtesy of: Kayed, R., Head, E., Thompson, J. L., McIntire, T. M., Milton, S. C., Cotman, C. W., et al. (2003). Common structure of soluble amyloid oligomers implies common mechanism of pathogenesis. Science 300, 486-489. doi: 10.1126/science.1079469.
Dot Blot: Amyloid Fibrils Antibody [NBP1-97929] - Dot blot analysis using Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929). Tissue: Cell lysates. Species: Human. Primary Antibody: Rabbit Anti-Amyloid Fibrils Fibrils (OC) Polyclonal Antibody (NBP1-97929) at 1:500, 1:5000. Beta Amyloid Fibrils HEPES-NaCl aggregation, showing 1:500 (L) and 1:5000 (R) time lapse dot blot.
Applications for Amyloid Fibrils Antibody - BSA Free
Application
Recommended Usage
Dot Blot
1:1000
ELISA
1:100-1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:100
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
1:10-1:500
Western Blot
1:1000
Application Notes
A 1:1000 dilution of Amyloid Fibrils (OC) Antibody was sufficient for detection of amyloid fibrils on PVDF membranes using transferred fibrils by colorimetric dot blot analysis using Goat anti-rabbit IgG:HRP as the secondary Antibody.
Reviewed Applications
Read 1 review rated 4 using NBP1-97929 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 50% Glycerol
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Product Documents for Amyloid Fibrils Antibody - BSA Free
Product Specific Notices for Amyloid Fibrils Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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