AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51175

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NBP3-51175-100ul
NBP3-51175-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB2660

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all AMPK alpha 1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Phospho-AMPK alpha 1 (S496) (Uniprot: Q13131)

Modification

p Ser496

Specificity

This antibody detects endogenous levels of PRKAA1 only when phosphorylated at S496

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AMPK alpha 1 [p Ser496] Antibody (23GB2660)

AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Immunocytochemistry/ Immunofluorescence: AMPK alpha 1 [p Ser496] Antibody (23GB2660)[NBP3-51175] -

Immunocytochemical staining of HeLa cells with AMPK alpha 1 antibody (1:1,000). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Flow Cytometry: AMPK alpha 1 [p Ser496] Antibody (23GB2660)[NBP3-51175] -

Flow cytometric analysis of AMPK alpha 1expression in HeLa cells using AMPK alpha 1 antibody (1:2,000). Green, isotype control; red, Phospho-AMPK alpha 1 (S496).
AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Western Blot: AMPK alpha 1 [p Ser496] Antibody (23GB2660)[NBP3-51175] -

Western blotting analysis using AMPK alpha 1 (S496) antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using FeQ(TM) ECL Substrate Kit.

Applications for AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: AMPK alpha 1

The 5'-AMP-activated protein kinase (AMPK), a member of the SNF1 (sucrose non-fermentor) kinase family (1) AMPK is a heterotrimeric protein comprising alpha (63 kDa), beta (38 kDa) and gamma (38 kDa) subunits. The alpha subunit is the catalytic subunit (which requires phosphorylation to be active) (2-3). Beta and gamma are non-catalytic subunits, although they have been found to interact with the active subunit in liver. AMPK regulates fatty acid and sterol synthesis by phosphorylation of acetyl-CoA as well as cholesterol synthesis via phosphorylation and inactivation of hydroxymethylglutaryl-CoA reductase (4). AMPK also appears to play a role in protecting cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways

Long Name

AMP-activated Protein Kinase alpha 1

Alternate Names

PRKAA1

Gene Symbol

PRKAA1

Additional AMPK alpha 1 Products

Product Documents for AMPK alpha 1 [p Ser496] Antibody (23GB2660)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for AMPK alpha 1 [p Ser496] Antibody (23GB2660)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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