Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI5F10

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all alpha-Methylacyl-CoA Racemase/AMACR Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human AMACR (NP_055139) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

42.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free (NBP2-45491) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)Azide and BSA Free [NBP2-71749]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)Azide and BSA Free [NBP2-71749]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free [NBP2-71749] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY AMACR.
Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free [NBP2-71749]

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free [NBP2-71749]

Immunohistochemistry: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free [NBP2-71749] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)Azide and BSA Free [NBP2-71749]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10)Azide and BSA Free [NBP2-71749]

Western Blot: alpha-Methylacyl-CoA Racemase/AMACR Antibody (OTI5F10) - Azide and BSA Free [NBP2-71749] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
View 1 more images

Applications

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: alpha-Methylacyl-CoA Racemase/AMACR

AMACR (alpha-methylacyl-CoA racemase) has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. Expression of AMACR protein is found in prostatic adenocarcinoma but not in benign prostatic tissue. It stains premalignant lesions of prostate: high-grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. AMACR can be used as a positive marker for PIN. Defects in AMACR are the cause of congenital bile acid synthesis defect type 4 (CBAS4); also known as cholestasis, intrahepatic, with defective conversion of trihydroxycoprostanic acid to cholic acid or trihydroxycoprostanic acid in bile. Clinical features include neonatal jaundice, intrahepatic cholestasis, bile duct deficiency and absence of cholic acid from bile.

Alternate Names

alphaMethylacylCoARacemase, AMACR, AMACRD, CBAS4, RACE

Gene Symbol

AMACR

Additional alpha-Methylacyl-CoA Racemase/AMACR Products

Product Documents

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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