alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44134

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL9772

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all alpha-Fetoprotein/AFP Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P02771, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free

alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Staining of human kidney shows moderate positivity in secretion.
alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Staining of human liver shows moderate positivity in secretion.
alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Immunohistochemistry-Paraffin: alpha-Fetoprotein/AFP Antibody [NBP3-44134]

Staining of human skeletal muscle shows no positivity as expected.
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Applications for alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: alpha-Fetoprotein/AFP

Alpha 1 Fetoprotein encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Alternate Names

AFP, DSCAM2

Gene Symbol

AFP

Additional alpha-Fetoprotein/AFP Products

Product Documents for alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for alpha-Fetoprotein/AFP Antibody (CL9772) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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