Alpha Amylase Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77711
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Bacteria
Applications
Western Blot, ELISA, Immunoprecipitation
Label
Biotin
Antibody Source
Polyclonal Rabbit IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Alpha Amylase [Bacillus amyloliquefaciens] (Uniprot: P00692)
Reactivity Notes
Reactive with Alpha Amylase from Bacillus amyloliquefaciens.
Cross reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined
Cross reactivity against Alpha Amylase from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified a-Amylase [B.amyloliquefaciens]
Scientific Data Images for Alpha Amylase Antibody [Biotin]
Western Blot: Alpha Amylase Antibody [Biotin] [NBP1-77711] - Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha Amylase antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Alpha Amylase. Other band(s): proteins within Alpha Amylase solution.
Alpha Amylase Antibody [Biotin]
Western Blot of Rabbit anti-Alpha Amylase Antibody Biotin Conjugated. Lane 1: Alpha Amylase solution. Load: 1/200 dilution of Alpha Amylase solution. Primary antibody: Alpha Amylase antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Alpha Amylase. Other band(s): proteins within Alpha Amylase solution.Applications for Alpha Amylase Antibody [Biotin]
Application
Recommended Usage
ELISA
1:5000 - 1:20000
Immunoprecipitation
1:100
Western Blot
1:500 - 1:5000
Application Notes
This product has been tested by western blot and is suitable to be assayed against 1.0 ug of a-Amylase in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Alpha Amylase
Alternate Names
AMY2;AMY2A;AMY2B;AMY3;HXA;PA
UniProt
Additional Alpha Amylase Products
Product Documents for Alpha Amylase Antibody [Biotin]
Product Specific Notices for Alpha Amylase Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...