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ALKBH8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38393

Novus Biologicals, part of Bio-Techne
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NBP3-38393-20ul
NBP3-38393-100ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human ALKBH8 (NP_620130.2).

Sequence:
MDSNHQSNYKLSKTEKKFLRKQIKAKHTLLRHEGIETVSYATQSLVVANGGLGNGVSRNQLLPVLEKCGLVDALLMPPNKPYSFARYRTTEESKRAYVTLNGKEVVDDLGQKITLYLNFVEKVQWKELRPQALPPGLMVVEEIISSEEEKMLLESVDWTEDTDNQNSQKSLKHRRVKHFGYEFHYENNNVDKDKPLSGGLPDICESFLEKWLRKGYIKHKPDQMTINQYE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit ALKBH8 Antibody - BSA Free (NBP3-38393) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for ALKBH8 Antibody - BSA Free

ALKBH8 Antibody

Western Blot: ALKBH8 Antibody [NBP3-38393] -

Western Blot: ALKBH8 Antibody [NBP3-38393] - Western blot analysis of various lysates, using ALKBH8 Rabbit pAb at 1:14000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
ALKBH8 Antibody

Immunohistochemistry: ALKBH8 Antibody [NBP3-38393] -

Immunohistochemistry: ALKBH8 Antibody [NBP3-38393] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using ALKBH8 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ALKBH8 Antibody

Immunohistochemistry: ALKBH8 Antibody [NBP3-38393] -

Immunohistochemistry: ALKBH8 Antibody [NBP3-38393] - Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using ALKBH8 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Applications for ALKBH8 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:100 - 1:200

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ALKBH8

ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis

Alternate Names

ABH8MGC10235, AlkB homologue 8, alkB, alkylation repair homolog 8 (E. coli), alkylated DNA repair protein alkB homolog 8, EC 1.14.11.-, EC 2.1.1.-, FLJ38204, Probable alpha-ketoglutarate-dependent dioxygenase ABH8, S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8

Gene Symbol

ALKBH8

Additional ALKBH8 Products

Product Documents for ALKBH8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ALKBH8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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