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Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37387

Novus Biologicals, part of Bio-Techne
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NBP2-37387
NBP2-37387-0.025ml

Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, ELISA, Flow Cytometry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3B10E7

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human Alkaline Phosphatase, Intestinal (AA: 397-458) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Mouse Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free (NBP2-37387) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7)BSA Free [NBP2-37387]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7)BSA Free [NBP2-37387]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mouse mAb against HL60 (1) and HepG2 (2) cell lysate.
Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free [NBP2-37387]

Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free [NBP2-37387]

Flow Cytometry: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Flow cytometric analysis of HepG2 cells using ALPI mouse mAb (green) and negative control (purple).
Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7)BSA Free [NBP2-37387]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7)BSA Free [NBP2-37387]

Western Blot: Alkaline Phosphatase, Intestinal Antibody (3B10E7) [NBP2-37387] - Western blot analysis using ALPI mAb against human ALPI recombinant protein. (Expected MW is 31.9 kDa)

Applications for Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Western Blot

1:500 - 1:2000
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Gene Symbol

ALPI

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody (3B10E7) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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