Alkaline Phosphatase, Bacterial Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB600-500
Key Product Details
Species Reactivity
E. coli
Applications
Validated:
Western Blot, ELISA
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Alkaline Phosphatase, Bacterial, Bacterial [E.coli] (Uniprot: P00634)
Reactivity Notes
Cross reactivity against Alkaline Phosphatase, Bacterial from other tissues and species may occur but have not been specifically determined. Some minimal cross reactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Specificity
Cross reactivity against Alkaline Phosphatase, Bacterial from other tissues and species may occur but have not been specifically determined. Some minimal cross reactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for Alkaline Phosphatase, Bacterial Antibody - BSA Free
Western Blot: Alkaline Phosphatase, Bacterial Antibody [Biotin] [NB600-500] - Lane 1: Alkaline Phosphatase (E. Coli). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (E. Coli) Antibody at 1:1,000 overnight at 4C. Secondary antibody: Peroxidase Rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for Alkaline Phosphatase (E. Coli).
Applications for Alkaline Phosphatase, Bacterial Antibody - BSA Free
Application
Recommended Usage
ELISA
1:4000-1:20000
Western Blot
1:500-1:2000
Application Notes
This product has been tested against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 of the reconstitution concentration is suggested for this product. A 1:10,000 dilution has been reported to be useful for the detection by immunoblot of an integral membrane protein from a gram-positive organism expressed as a LacZ/PhoA chimeric derivative in E.coli. The researcher should determine optimal titers for other applications.
Reviewed Applications
Read 1 review rated 4 using NB600-500 in the following applications:
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase, Bacterial
Additional Alkaline Phosphatase, Bacterial Products
Product Documents for Alkaline Phosphatase, Bacterial Antibody - BSA Free
Product Specific Notices for Alkaline Phosphatase, Bacterial Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...