Alkaline Phosphatase/ALPP Antibody (24GB5430)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51713
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 24GB5430
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthesized peptide derived from human Alkaline Phosphatase/ALPP (Uniprot: P05187)
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (24GB5430)
Immunocytochemistry/ Immunofluorescence: Alkaline Phosphatase/ALPP Antibody (24GB5430)[NBP3-51713] -
Immunocytochemical staining of HepG2 cells with Alkaline Phosphatase/ALPP antibody (1:1,000). Nuclei were stained blue with DAPI; Alkaline Phosphatase/ALPP was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Flow Cytometry: Alkaline Phosphatase/ALPP Antibody (24GB5430)[NBP3-51713] -
Flow cytometric analysis of Alkaline Phosphatase/ALPPexpression in HepG2 cells using Alkaline Phosphatase/ALPP antibody (1:2,000). Green, isotype control; red, ALPP.
Western Blot: Alkaline Phosphatase/ALPP Antibody (24GB5430)[NBP3-51713] -
Western blotting analysis using Alkaline Phosphatase/ALPP antibody (1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.Applications for Alkaline Phosphatase/ALPP Antibody (24GB5430)
Application
Recommended Usage
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.4), 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (24GB5430)
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (24GB5430)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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