Alkaline Phosphatase/ALPL Antibody (24GB5855)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-51745

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NBP3-51745-100ul
NBP3-51745-20ul
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 24GB5855

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Alkaline Phosphatase/ALPL Antibodies »

Product Specifications

Immunogen

Recombinant protein of human Alkaline Phosphatase/ALPL (Uniprot: P05186)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Alkaline Phosphatase/ALPL Antibody (24GB5855)

Alkaline Phosphatase/ALPL Antibody (24GB5855)

Flow Cytometry: Alkaline Phosphatase/ALPL Antibody (24GB5855)[NBP3-51745] -

Flow cytometric analysis of Alkaline Phosphatase/ALPLexpression in HepG2 cells using Alkaline Phosphatase/ALPL antibody (1:2,000). Green, isotype control; red, ALPL.
Alkaline Phosphatase/ALPL Antibody (24GB5855)

Flow Cytometry: Alkaline Phosphatase/ALPL Antibody (24GB5855)[NBP3-51745] -

Validation of Alkaline Phosphatase/ALPL knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Alkaline Phosphatase/ALPL antibody (1:2,000) and analyzed using BD flow cytometer.
Alkaline Phosphatase/ALPL Antibody (24GB5855)

Western Blot: Alkaline Phosphatase/ALPL Antibody (24GB5855)[NBP3-51745] -

Western blotting analysis using Alkaline Phosphatase/ALPL antibody (1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (1:20,000) respectively. Image was developed using NaQ(TM) ECL Substrate Kit.
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Applications for Alkaline Phosphatase/ALPL Antibody (24GB5855)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase/ALPL

Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.

Long Name

Alkaline Phosphatase Liver

Alternate Names

Akp2, AP-TNAP, HOPS, TNAP, TNSALP

Gene Symbol

ALPL

Additional Alkaline Phosphatase/ALPL Products

Product Documents for Alkaline Phosphatase/ALPL Antibody (24GB5855)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Alkaline Phosphatase/ALPL Antibody (24GB5855)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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