African Swine Fever Virus p54 Antibody (GT1075) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13672
Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Lateral Flow Assay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GT1075
Format
Azide and BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of African Swine Fever Virus p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.
Reactivity Notes
African swine fever virus
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Description
Centrifuge briefly prior to opening.
Scientific Data Images for African Swine Fever Virus p54 Antibody (GT1075) - Azide and BSA Free
![Western Blot: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]](https://resources.bio-techne.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT1075-Western-Blot-NBP3-13672-img0001.jpg "Western Blot: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]")
Western Blot: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]
Western Blot: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.![Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]](https://resources.bio-techne.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT1075-Immunocytochemistry-Immunofluorescence-NBP3-13672-img0002.jpg "Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]")
Immunocytochemistry/ Immunofluorescence: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]
Immunocytochemistry/Immunofluorescence: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672] - African Swine Fever Virus p54 antibody [GT1075] detects African Swine Fever Virus p54 protein by immunofluorescent analysis. Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) diluted at 1:1000. Blue: Fluoroshield with DAPI.![Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]](https://resources.bio-techne.com/images/products/African-Swine-Fever-Virus-p54-Antibody-GT1075-Immunohistochemistry-Paraffin-NBP3-13672-img0003.jpg "Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]")
Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672]
Immunohistochemistry-Paraffin: African Swine Fever Virus p54 Antibody (GT1075) [NBP3-13672] - African Swine Fever Virus p54 antibody [GT1075] detects African Swine Fever Virus p54 protein at cytoplasm by immunohistochemical analysis. Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block. Green: African Swine Fever Virus p54 stained by African Swine Fever Virus p54 antibody [GT1075] (NBP3-13672) diluted at 1:1000. Blue: Fluoroshield with DAPI. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for African Swine Fever Virus p54 Antibody (GT1075) - Azide and BSA Free
Application
Recommended Usage
ELISA
Assay dependent
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Lateral Flow Assay
Assay dependent
Sandwich ELISA
Assay dependent
Western Blot
1:1000-1:10000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS, 20% Glycerol
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: African Swine Fever Virus p54
Alternate Names
ASFV p54, Ba71V-126, E183L, Envelope protein p54, Structural protein p54
Gene Symbol
E183L
Additional African Swine Fever Virus p54 Products
Product Documents for African Swine Fever Virus p54 Antibody (GT1075) - Azide and BSA Free
Product Specific Notices for African Swine Fever Virus p54 Antibody (GT1075) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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