AFAP1L2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06316
![Western Blot: AFAP1L2 Antibody [NBP3-06316]](https://resources.bio-techne.com/images/products/AFAP1L2-Antibody-Western-Blot-NBP3-06316-img0001.jpg "Western Blot: AFAP1L2 Antibody [NBP3-06316]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human AFAP1L2 fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for AFAP1L2 Antibody - BSA Free
Western Blot: AFAP1L2 Antibody [NBP3-06316]
Western Blot: AFAP1L2 Antibody [NBP3-06316] - Anti-AFAP1L2 rabbit polyclonal antibody at 1:500 dilutionLane A: ACHN Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size: 91 kDa![Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316]](https://resources.bio-techne.com/images/products/AFAP1L2-Antibody-Immunoprecipitation-NBP3-06316-img0002.jpg "Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316]")
Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316]
Immunoprecipitation: AFAP1L2 Antibody [NBP3-06316] - AFAP1L2 was immunoprecipitated using:Lane A: 0.5 mg U-251 MG Whole Cell Lysate4 uL anti-AFAP1L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG. Primary antibody:Anti-AFAP1L2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 91 kDaObserved band size :100 kDa
Immunocytochemistry/Immunofluorescence: AFAP1L2 Antibody [NBP3-06316] -
Immunocytochemistry/Immunofluorescence: AFAP1L2 Antibody [NBP3-06316] - Immunofluorescence staining of AFAP1L2 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06316 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.Applications for AFAP1L2 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Format
BSA Free
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AFAP1L2
Alternate Names
actin filament associated protein 1-like 2, actin filament-associated protein 1-like 2, AFAP1-like protein 2, FLJ14564, KIAA1914Em:AC005383.4, XB130CTB-1144G6.4
Gene Symbol
AFAP1L2
Additional AFAP1L2 Products
Product Documents for AFAP1L2 Antibody - BSA Free
Product Specific Notices for AFAP1L2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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