ADE2 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90235
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0010.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]")
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0015.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Validated by
Orthogonal Validation, Independent Antibodies
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Hamster - Cricetulus (Chinese Hamster)
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Simple Western
Cited:
Western Blot, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: ETAFIAPQCEMIPIEWVCRRIATGSFLKRNPGVKEGYKFYPPKVELFFKDDANNDPQWSEEQLIAAKFCFAGLLIGQTEVDIMS
Reactivity Notes
Chinese Hamster reactivity reported in scientific literature (PMID: 23394948).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ADE2 Antibody - BSA Free
![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0011.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]")
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0012.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]")
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]
Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235] - Staining of human skeletal muscle shows very weak positivity in myocytes as expected.![Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Immunohistochemistry-Paraffin-NBP1-90235-img0013.jpg "Immunohistochemistry-Paraffin: ADE2 Antibody [NBP1-90235]")
![Simple Western: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Simple-Western-NBP1-90235-img0006.jpg "Simple Western: ADE2 Antibody [NBP1-90235]")
![ADE2 Antibody - BSA Free Western Blot: ADE2 Antibody - BSA Free [NBP1-90235]](https://resources.bio-techne.com/images/products/nbp1-90235_rabbit-polyclonal-ade2-antibody-30520257263821.jpg "Western Blot: ADE2 Antibody - BSA Free [NBP1-90235]")
![Western Blot: ADE2 Antibody [NBP1-90235]](https://resources.bio-techne.com/images/products/ADE2-Antibody-Western-Blot-NBP1-90235-img0009.jpg "Western Blot: ADE2 Antibody [NBP1-90235]")
Applications for ADE2 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Simple Western
1:20
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 47, 50 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:20, apparent MW was 47, 50 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ADE2
Alternate Names
ADE2, AIR carboxylase, AIRCADE2H1, DKFZp781N1372, MGC1343, MGC5024, multifunctional protein ADE2, PAIS, phosphoribosylaminoimidazole carboxylase, phosphoribosylaminoimidazolesuccinocarboxamide synthetase, SAICAR synthetase
Entrez Gene IDs
10606 (Human)
Gene Symbol
PAICS
UniProt
Additional ADE2 Products
Product Documents for ADE2 Antibody - BSA Free
Product Specific Notices for ADE2 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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