active/cleaved Caspase-9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48628

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NBP3-48628
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all active/cleaved Caspase-9 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human active/cleaved Caspase-9 (cleaved Asp330). The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for active/cleaved Caspase-9 Antibody

active/cleaved Caspase-9 Antibody

Western Blot: active/cleaved Caspase-9 Antibody [NBP3-48628] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with active/cleaved Caspase-9 antibody (NBP3-48628) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
active/cleaved Caspase-9 Antibody

Western Blot: active/cleaved Caspase-9 Antibody [NBP3-48628] -

Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with active/cleaved Caspase-9 antibody (NBP3-48628) diluted at 1:500.

Applications for active/cleaved Caspase-9 Antibody

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: active/cleaved Caspase-9

Caspase 9 (CASP9) is a member of the cysteine-aspartic acid protease (caspase) family that is involved in the execution-phase of cell apoptosis. Caspase 9 is expressed as an inactive proenzyme which then undergos proteolytic processing by upstream caspases, to form an active (cleaved) caspase 9 form.

Alternate Names

APAF-3APAF3, apoptotic protease activating factor 3, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, CASP-9, caspase 9, apoptosis-related cysteine peptidase, caspase-9, EC 3.4.22.62, ICE-LAP6CASPASE-9c, ICE-like apoptotic protease 6, MCH6apoptosis-related cysteine protease

Additional active/cleaved Caspase-9 Products

Product Documents for active/cleaved Caspase-9 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for active/cleaved Caspase-9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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